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Wednesday, January 15, 2014

Huge Advance In Diagnosis Of Malaria

Plasmodium Falciparum (stained green)
amidst red blood cells

***

Malaria
Wikipedia

200,000,000 people infected yearly.
Of those, c. 1,000,000 die.

Needle-Free Transdermal Malaria Diagnosis 
Richard T. Ellison III, MD


A novel transdermal laser technique can detect malaria noninvasively, 
without the need for chemical reagents or blood samples.
http://www.jwatch.org/content/2014/NA33366?query=etoc_jwid


The need for sophisticated blood testing for diagnosis has hampered efforts
to control malaria. However, all blood-stage malaria parasites produce 
nanocrystals of a unique compound — hemozoin — as they digest hemoglobin, 
and researchers have now developed a technique that appears to be able to 
detect these nanoparticles transdermally.


    Hemozoin has high optical absorbance, and the heat generated when 
    hemozoin nanocrystals are exposed to a picosecond pulse from a 
    near-infrared laser evaporates liquid around them, producing transient 
    vapor nanobubbles (VNBs). The explosive generation and subsequent 
    collapse of these VNBs generate characteristic optical and acoustic 
    signals, and researchers were able to define these signals in studies 
    of isolated hemozoin nanocrystals in water. Subsequent experiments 
    confirmed that such VNBs could be generated and detected within 
    Plasmodium falciparum–infected human red blood cells, but not in 
    uninfected red blood cells. In studies involving malaria-infected mice,
    a transdermal laser probe applied to the ear could detect hemozoin VNBs
    at a 0.00034% level of parasitemia without any apparent discomfort or 
    morphological damage to ear skin or blood vessels.


      Comment
      This technique needs to be tested in humans to define its 
      sensitivity, specificity, and safety. However, assuming no safety 
      concerns, it could potentially revolutionize the diagnosis of 
      malaria and greatly improve clinical management, as well as 
      surveillance and eradication efforts.

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